Ferric reducing antioxidant power (FRAP) assay is carried out using the earlier reported method as described by Benzie and Strain (1996). Antioxidant activities were evaluated by four assays: an antioxidant activity assay using Saccharomyces cerevisiae, a DPPH ((2, 2-diphenyl-1-picrylhydrazyl) assay to assess free radical scavenging, an assay assessing ferrous ions or iron (II) chelating ability, and a ferric reducing antioxidant power (FRAP) assay.Total phenolic and flavonoid contents were 1, and was determined using a methanol solution of reagent DPPH in the presence of a hydrogen donor molecule. However, SFE extracts showed high antioxidant activity comparable to the ethanol 80% extract (polyphenol, DPPH, and reducing power method). Methods. File Type PDF Antioxidant Activity And Physicochemical .Salve Antioxidant Assay Principle \u0026 Process (DPPH \u0026 H2O2): Dr. Bhushan P Pimple Antidotes and the Clinical Applications Ferric Reducing Antioxidant Power (FRAP) assay \\\\ Antioxidant activity of plant extracts #Medicinal chemistry#Protein binding\u0026Chelation However, SFE extracts showed high antioxidant activity comparable to the ethanol 80% extract (polyphenol, DPPH, and reducing power method). While the FRAP assay showed a low iron-reducing power values for both extracts compared to BHT), the overall antioxidant activity of the two extracts was found to be considerable. The wavelength depends on the antioxidant power assay your are carrying out, it is not a universal one. For instance, in Ferric Reducing Antioxidant Power Assay (FRAP) assay, the wavelength to detect the reduced compound is 595 nm. Ferric Ion Reducing Antioxidant Power (FRAP) Assay. The average scavenging DPPH radical activity, reducing power. Flow-Through Chemiluminescence (FTCL) Assay. Share sensitive information only on official, secure websites. The antioxidant activity of the various DS extracts of the variety "Deglet Nour", is shown in Fig. Phytochemical Screening and in vitro Antioxidant Activity of Jawarish Amla- A Poly Herbal Formulation. Furthermore, the antimicrobial assay showed the strongest effect with SWG.70. Ferric Reducing Antioxidant Power This is a sensitive and simple technique used regularly to evaluate the antioxidant capacity of biological fluids, diet components, and supplements. Ferric Ion Reducing Antioxidant Power (FRAP) Assay. Ferric Reducing Antioxidant Power (FRAP) Activity. Antibacterial and antioxidant activity of oregano. A FRAP assay treats the antioxidants as reductants in a redox linked colorimetric reaction, with the value reflecting the reducing power of antioxidants, which is used for a rapid measurement of the total antioxidant capacity of the sample . The wavelength depends on the antioxidant power assay your are carrying out, it is not a universal one. 2.5.2. Antioxidant activity. 2. The best synergistic effect between caffeic acid and SAC based on DPPH, ABTS, and reducing power assays were observed at the ratio of 1:20, 1:35, and 1:70, respectively. , with a slight modification. The average scavenging DPPH radical activity, reducing power. The essential oil exhibited good radical scavenging activity against DPPH, ABTS, and Reducing power assay with half-maximal inhibitory concentration (IC50) values of 6. The clinical utility of measuring antioxidant, a-tocopherol-, ascorbic acid-, and bilirubin-related ab- or reducing, power in biological fluids remains to be sorbance changes. The assay is based on measuring the decrease of the absorbance of DPPH radical (at a wavelength of 517 nm) as a result of its reaction with antioxidants from the sample. Compounds with reducing power indicate that they are electron donors and can reduce the oxidized intermediates of lipid peroxidation processes, so that they can act as primary and secondary antioxidants8. Catalog Number: STA-859. Albumin reacts very slowly and its established. several in vitro radical scavenging and reducing power assessment assays such as 2,2-diphenyl-1-picrylhydrazyl (dpph), 2,2-azino-bis (3-ethylbenzothiazoline-6 Chemical Composition, Antioxidant, Anti-inflammatory and Anticancer Activities of Bark Essential Oil of Cryptocarya amygdalina from India. The clinical utility of measuring antioxidant, a-tocopherol-, ascorbic acid-, and bilirubin-related ab- or reducing, power in biological fluids remains to be sorbance changes. At low cost, this method showed to be useful for screening of antioxidant capacities and comparing e ciencies of di erent compounds. Search: Bulk Peptides. Antioxidants that react in the FRAP assay are those that can reduce, under the reaction conditions used, the Fe 3+- TPTZ salt to its blue colored Fe 2+- TPTZ form. The use of antioxidants in vegetable oils A review. Earn Free Access Learn More > Upload Documents 1, and was determined using a methanol solution of reagent DPPH in the presence of a hydrogen donor molecule. You can ask !. The synthesized chalcones (3a-3d) were evaluated for in vitro radical scavenging activity using diphenylpicrylhydrazyl (DPPH) model as well as antioxidant activity using Fe reducing power capacity. A simple, automated test measuring the ferric reducing ability of plasma, the FRAP assay, is presented as a novel method for assessing "antioxidant power." This study provides some essential further evidence on this point based on the reported data and mechanisms underlying the antioxidant functions as well as the anodic oxidation of Methods. The reducing power of a compound is related to its electron transfer ability and may serve as a significant indicator of its potential antioxidant activity. OxiSelect Ferric Reducing Antioxidant. In this study, three kinds of mushroom polysaccharides, including Lentinus edodes, Lentinula edodes and Agaricus bisporus, were investigated for their antioxidant effects using the 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical-scavenging system and ferric reducing/antioxidant power (FRAP) assay. The tests based on the transfer of one electron include the Cupric Reducing Antioxidant Power (CUPRAC) test, the Ferric Reducing Antioxidant Power (FRAP) test, the FolinCiocalteu test. Assay Principle: The OxiSelect Ferric Reducing Antioxidant Power (FRAP) Assay Kit is a quantitative assay for measuring the antioxidant potential within a sample. The reducing power of a compound is related to its electron transfer ability and may serve as a significant indicator of its potential antioxidant activity. This method was criticized for lacking standardization in different stages of the analytical process [ 37 ]. Station Cl. Ferric to ferrous ion reduction at low pH causes a colored ferrous-tripyridyltriazine complex to form. Abstract Electrochemical assay has been employed recently to study the activity of antioxidants; however, there is controversy as to whether reducing power fully characterizes the antioxidant activity. Lipid Peroxidation Assay Using Rat Brain Tissue. Human LDL Oxidation Assay. Antioxidant activities were evaluated by four assays: an antioxidant activity assay using Saccharomyces cerevisiae, a DPPH ((2, 2-diphenyl-1-picrylhydrazyl) assay to assess free radical scavenging, an assay assessing ferrous ions or iron (II) chelating ability, and a ferric reducing antioxidant power (FRAP) assay.Total phenolic and flavonoid contents were 0 downloads 0 Views 807KB Size. Furthermore, the antimicrobial assay showed the strongest effect with SWG.70. DPPH Radical Cation Scavenging Assay. FRAP and TRAP Assays The ferric ion reducing antioxidant power (FRAP) assay is a non-specific, [10]. Abstract. Material and methods 2.1. Superoxide Radical Scavenging Assay
These include ascorbic acid (vitamin C), -tocopherol (vitamin E), uric acid, bilirubin, and polyphenolic compounds such as catechins and other flavonoids in plant-based foods.
ABTS+ Radical Cation Scavenging Assay. When ferric chloride reacts with 2,4,6-tripyridyl- s -triazine (TPTZ) at low pH, ferric is converted into ferrous causing formation of ferrous tripyridyl triazine complex. The reducing power of various essential oils was conducted according to Oyaizu and Ferreira et al. , with a slight modification. The antioxidant power of the EOs tested was estimated by comparison with a natural antioxidant (ascorbic acid). But ORAC measures antioxidant activity in a test tube, not in the human body. Ferric Ion Reducing Antioxidant Power (FRAP) Assay. The total antioxidant capacity was controlled by phosphomolybdate assay, and the antimicrobial effect of single-bulb black garlic samples was tested in vitro. Antioxidant activity. Antioxidant Activities; Assay Reducing Power; Learn more from Antioxidant Activities Manuscript Generator Sentences Filter. Welcome to Kapabio Systems! Benzie I.F.F., Strain J.J. Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol. 1999;299:1527. reducing power and antioxidant activity. But ORAC measures antioxidant activity in a test tube, not in the human body. CARUM COPTICUM ESSENTIAL OILS AS NATURAL ANTIOXIDANT IN. Our skin care products work for all skin types like dry skin, oily skin, normal skin, sensitive skin, and a combination of them One 2008 study found that consuming gelatin (the cooked form of collagen) increased participants' levels of the satiety hormone, ghrelin All of our products are wholesale pricing perfect for anyone who wants to start But ORAC measures antioxidant activity in a test tube, not in the human body. Reducing power and antioxidant assays were carried out using 1-diphenyl-2-picryl hydrazyl radical (DPPH) and H 2 O 2. Earn . The antioxidant power of the EOs tested was estimated by comparison with a natural antioxidant (ascorbic acid). The antioxidant activities of the SBG samples agreed with the antioxidant components assessed using DPPH, H 2 O 2, reducing power, and total antioxidant activity. The three antioxidant activity assays in our study included DPPH, ABTS, and reducing power assays, which belong to electron transfer assays [ 20, 21, 22 ]. FRAP and TRAP Assays The ferric ion reducing antioxidant power (FRAP) assay is a non-specific, [10]. A locked padlock) or https:// means youve safely connected to the .gov website. The antioxidant power of the EOs tested was estimated by comparison with a natural antioxidant (ascorbic acid). Albumin reacts very slowly and its established. Increasing Antioxidant Activity and Reducing Decay of. Methods. , with a slight modification. But ORAC measures antioxidant activity in a test tube, not in the human body. The antioxidant trend for the ABTS assay was different from the DPPH assay, with the total antioxidant activity ranging from EC 50 values of 6.06 to 69.19 g/mL for methanol extracts, 5.79 to 145.90 g/mL for water extracts, 3.09 to 258.40 g/mL for dichloromethane extracts, and 5.81 to 1397 g/mL for the essential oils. Table 2. The antioxidant trend for the ABTS assay was different from the DPPH assay, with the total antioxidant activity ranging from EC 50 values of 6.06 to 69.19 g/mL for methanol extracts, 5.79 to 145.90 g/mL for water extracts, 3.09 to 258.40 g/mL for dichloromethane extracts, and 5.81 to 1397 g/mL for the essential oils. 20 In this assay, the yellow color of the test solution changes to green and blue depending on the reducing power of test substance. Ferric Reducing-Antioxidant Power (FRAP) Assay.
Ferric Reducing Antioxidant Power assay (FRAP) [17] is based on reduction of a colorless Fe3+-TPTZ complex into intense blue Fe2+-TPTZ once it interacts with a potential antioxidant. Antioxidant activities were evaluated by four assays: an antioxidant activity assay using Saccharomyces cerevisiae, a DPPH ((2, 2-diphenyl-1-picrylhydrazyl) assay to assess free radical scavenging, an assay assessing ferrous ions or iron (II) chelating ability, and a ferric reducing antioxidant power (FRAP) assay.Total phenolic and flavonoid contents were The ferric reducing antioxidant power (FRAP) assay is a typical ET-based method that measures the reduction of ferric ion (Fe3+)-ligand complex to the intensely blue-colored ferrous (Fe2+) complex by antioxidants in an acidic medium. 4 6 All statistical analyses were performed with the statistical package SAS for Windows, version 6.12 (SAS Institute Inc., USA).
, Potters Bar EN6 1TL, United Kingdom 020 3393 8531; Sign in or Register Essential oils as antioxidants their evaluation by DPPH.
Antioxidant activity (DPPH radical activity, reducing power, SOA activity, total phenolic content and total flavonoid content) were evaluated in Indian variety of acerola and its squash. Nevertheless, the FRAP assay appears to activity is low. The ferric reducing antioxidant power (FRAP) assay is a typical ET-based method that measures the reduction of ferric ion (Fe 3+ )-ligand complex to the intensely blue-colored ferrous (Fe 2+) complex by antioxidants in an acidic medium. 2.5.2. Nevertheless, the FRAP assay appears to activity is low. ORAC Scores measure Antioxidant Power of Essential Oils. Antioxidant activity is determined as increase of absorbance at 593 nm, and results are expressed as micromolar Fe 2+ equivalents or relative to Albumin reacts very slowly and its established. and reducing power antioxidant assay (RPAA). The antioxidant activity of the various DS extracts of the variety "Deglet Nour", is shown in Fig. Blood and Plasma Total Antioxidant Capacity (TAC) Assay. Nevertheless, the FRAP assay appears to activity is low. Apigenin Brain Apigenin is an antioxidant compound found in a wide variety of plants and herbs. The reducing power of various essential oils was conducted according to Oyaizu and Ferreira et al. This activity was then compared with an antioxidant reference, 2,6-di-tertbutyl-4-methyl phenol (BHT).
This activity was then compared with an antioxidant reference, 2,6-di-tertbutyl-4-methyl phenol (BHT). 2.5.2. The antioxidant activities of the SBG samples agreed with the antioxidant components assessed using DPPH, H 2 O 2, reducing power, and total antioxidant activity. Author: Felix Dixon. Methods Enzymol. In this assay, the yellow color of the test solution changes to green and blue depending on the reducing power of test sample. Reducing power is associated with antioxidant activity and may serve as a significant reflection of the antioxidant activity7. Oxidative stress markers albumin (A), uric acid (B), total antioxidant capacity (TAC) determined by ABTS cation inhibition method alone (ABTS, C) or in association with peroxidase (ABTS + HRP, D), cupric reducing antioxidant capacity assay (CUPRAC, E) and by ferric reducing antioxidant power assay (FRAP, F), total oxidant capacity (TOC, G) and lipid peroxidation The clinical utility of measuring antioxidant, a-tocopherol-, ascorbic acid-, and bilirubin-related ab- or reducing, power in biological fluids remains to be sorbance changes. In this study, three kinds of mushroom polysaccharides, including Lentinus edodes, Lentinula edodes and Agaricus bisporus, were investigated for their antioxidant effects using the 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical-scavenging system and ferric reducing/antioxidant power (FRAP) assay. When the F-ratio of ANOVA was significant, the contribution of reducing power to antioxidant activity was assessed by the least significant difference method ( P < 0.05). We present experimental and theoretical studies on the antioxidant potential of the set of 22 phenolic acids with different models of (PDF) Antioxidant Activity of Selected Phenolic AcidsFerric Reducing Antioxidant Power Assay and QSAR Analysis of the Structural Features | Beata bikowska - Academia.edu A locked padlock) or https:// means youve safely connected to the .gov website. The best synergistic effect between caffeic acid and SAC based on DPPH, ABTS, and reducing power assays were observed at the ratio of 1:20, 1:35, and 1:70, respectively. Due to the high antioxidant activity of the tested fractions, their cytotoxic activity was evaluated via using preliminary brine shrimp lethality test and toward liver cancer cell line; HepG2 using Sulphorhodamine-B assay. Chemicals Antioxidant activity of BHT was measured by DPPH radical scavenging, iron reducing power and ABTS assay was shown in Table 2. The reducing power of various essential oils was conducted according to Oyaizu and Ferreira et al. FRAP assay by coulometric titration has been attractive as a highly sensitive, reliable, and simple test for reducing power evaluation. Another electrochemical version of the FRAP assay is the chronoamperometric measurement of antioxidant reducing power. Subsequently, the antioxidant activity was demonstrated by 2, 2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging assay, reducing power Thus far, various chemical tests coupled with highly sensitive and automated detection technologies have been used to evaluate antioxidant activity by special methods, like for instance scavenging activity against different types of free radicals or ROS, reducing power and metal chelation, among others. In this technique, ferric iron complexed to 2,4,6-tri(2-pyridyl)-1,3,5-triazine (TPTZ, Scheme 1 ) acts both as the oxidant and chromophore 2 [ 13 ]. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Share sensitive information only on official, secure websites. Electrochemical assay has been employed recently to study the activity of antioxidants; however, there is controversy as to whether reducing power fully characterizes the antioxidant activity. Antioxidant activity (DPPH radical activity, reducing power, SOA activity, total phenolic content and total flavonoid content) were evaluated in Indian variety of acerola and its squash. Reducing power assay The antioxidant activity of altered crude extracts was evaluated using the reducing power assay. However, in DPPH and ABTS assays, factors such as light, oxygen, and pH easily influence the color of the reactants mixture.
These include ascorbic acid (vitamin C), -tocopherol (vitamin E), uric acid, bilirubin, and polyphenolic compounds such as catechins and other flavonoids in plant-based foods.
ABTS+ Radical Cation Scavenging Assay. When ferric chloride reacts with 2,4,6-tripyridyl- s -triazine (TPTZ) at low pH, ferric is converted into ferrous causing formation of ferrous tripyridyl triazine complex. The reducing power of various essential oils was conducted according to Oyaizu and Ferreira et al. , with a slight modification. The antioxidant power of the EOs tested was estimated by comparison with a natural antioxidant (ascorbic acid). But ORAC measures antioxidant activity in a test tube, not in the human body. Ferric Ion Reducing Antioxidant Power (FRAP) Assay. The total antioxidant capacity was controlled by phosphomolybdate assay, and the antimicrobial effect of single-bulb black garlic samples was tested in vitro. Antioxidant activity. Antioxidant Activities; Assay Reducing Power; Learn more from Antioxidant Activities Manuscript Generator Sentences Filter. Welcome to Kapabio Systems! Benzie I.F.F., Strain J.J. Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol. 1999;299:1527. reducing power and antioxidant activity. But ORAC measures antioxidant activity in a test tube, not in the human body. CARUM COPTICUM ESSENTIAL OILS AS NATURAL ANTIOXIDANT IN. Our skin care products work for all skin types like dry skin, oily skin, normal skin, sensitive skin, and a combination of them One 2008 study found that consuming gelatin (the cooked form of collagen) increased participants' levels of the satiety hormone, ghrelin All of our products are wholesale pricing perfect for anyone who wants to start But ORAC measures antioxidant activity in a test tube, not in the human body. Reducing power and antioxidant assays were carried out using 1-diphenyl-2-picryl hydrazyl radical (DPPH) and H 2 O 2. Earn . The antioxidant power of the EOs tested was estimated by comparison with a natural antioxidant (ascorbic acid). The antioxidant activities of the SBG samples agreed with the antioxidant components assessed using DPPH, H 2 O 2, reducing power, and total antioxidant activity. The three antioxidant activity assays in our study included DPPH, ABTS, and reducing power assays, which belong to electron transfer assays [ 20, 21, 22 ]. FRAP and TRAP Assays The ferric ion reducing antioxidant power (FRAP) assay is a non-specific, [10]. A locked padlock) or https:// means youve safely connected to the .gov website. The antioxidant power of the EOs tested was estimated by comparison with a natural antioxidant (ascorbic acid). Albumin reacts very slowly and its established. Increasing Antioxidant Activity and Reducing Decay of. Methods. , with a slight modification. But ORAC measures antioxidant activity in a test tube, not in the human body. The antioxidant trend for the ABTS assay was different from the DPPH assay, with the total antioxidant activity ranging from EC 50 values of 6.06 to 69.19 g/mL for methanol extracts, 5.79 to 145.90 g/mL for water extracts, 3.09 to 258.40 g/mL for dichloromethane extracts, and 5.81 to 1397 g/mL for the essential oils. Table 2. The antioxidant trend for the ABTS assay was different from the DPPH assay, with the total antioxidant activity ranging from EC 50 values of 6.06 to 69.19 g/mL for methanol extracts, 5.79 to 145.90 g/mL for water extracts, 3.09 to 258.40 g/mL for dichloromethane extracts, and 5.81 to 1397 g/mL for the essential oils. 20 In this assay, the yellow color of the test solution changes to green and blue depending on the reducing power of test substance. Ferric Reducing-Antioxidant Power (FRAP) Assay.
Ferric Reducing Antioxidant Power assay (FRAP) [17] is based on reduction of a colorless Fe3+-TPTZ complex into intense blue Fe2+-TPTZ once it interacts with a potential antioxidant. Antioxidant activities were evaluated by four assays: an antioxidant activity assay using Saccharomyces cerevisiae, a DPPH ((2, 2-diphenyl-1-picrylhydrazyl) assay to assess free radical scavenging, an assay assessing ferrous ions or iron (II) chelating ability, and a ferric reducing antioxidant power (FRAP) assay.Total phenolic and flavonoid contents were The ferric reducing antioxidant power (FRAP) assay is a typical ET-based method that measures the reduction of ferric ion (Fe3+)-ligand complex to the intensely blue-colored ferrous (Fe2+) complex by antioxidants in an acidic medium. 4 6 All statistical analyses were performed with the statistical package SAS for Windows, version 6.12 (SAS Institute Inc., USA).
, Potters Bar EN6 1TL, United Kingdom 020 3393 8531; Sign in or Register Essential oils as antioxidants their evaluation by DPPH.
Antioxidant activity (DPPH radical activity, reducing power, SOA activity, total phenolic content and total flavonoid content) were evaluated in Indian variety of acerola and its squash. Nevertheless, the FRAP assay appears to activity is low. The ferric reducing antioxidant power (FRAP) assay is a typical ET-based method that measures the reduction of ferric ion (Fe 3+ )-ligand complex to the intensely blue-colored ferrous (Fe 2+) complex by antioxidants in an acidic medium. 2.5.2. Nevertheless, the FRAP assay appears to activity is low. ORAC Scores measure Antioxidant Power of Essential Oils. Antioxidant activity is determined as increase of absorbance at 593 nm, and results are expressed as micromolar Fe 2+ equivalents or relative to Albumin reacts very slowly and its established. and reducing power antioxidant assay (RPAA). The antioxidant activity of the various DS extracts of the variety "Deglet Nour", is shown in Fig. Blood and Plasma Total Antioxidant Capacity (TAC) Assay. Nevertheless, the FRAP assay appears to activity is low. Apigenin Brain Apigenin is an antioxidant compound found in a wide variety of plants and herbs. The reducing power of various essential oils was conducted according to Oyaizu and Ferreira et al. This activity was then compared with an antioxidant reference, 2,6-di-tertbutyl-4-methyl phenol (BHT).
This activity was then compared with an antioxidant reference, 2,6-di-tertbutyl-4-methyl phenol (BHT). 2.5.2. The antioxidant activities of the SBG samples agreed with the antioxidant components assessed using DPPH, H 2 O 2, reducing power, and total antioxidant activity. Author: Felix Dixon. Methods Enzymol. In this assay, the yellow color of the test solution changes to green and blue depending on the reducing power of test sample. Reducing power is associated with antioxidant activity and may serve as a significant reflection of the antioxidant activity7. Oxidative stress markers albumin (A), uric acid (B), total antioxidant capacity (TAC) determined by ABTS cation inhibition method alone (ABTS, C) or in association with peroxidase (ABTS + HRP, D), cupric reducing antioxidant capacity assay (CUPRAC, E) and by ferric reducing antioxidant power assay (FRAP, F), total oxidant capacity (TOC, G) and lipid peroxidation The clinical utility of measuring antioxidant, a-tocopherol-, ascorbic acid-, and bilirubin-related ab- or reducing, power in biological fluids remains to be sorbance changes. In this study, three kinds of mushroom polysaccharides, including Lentinus edodes, Lentinula edodes and Agaricus bisporus, were investigated for their antioxidant effects using the 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical-scavenging system and ferric reducing/antioxidant power (FRAP) assay. When the F-ratio of ANOVA was significant, the contribution of reducing power to antioxidant activity was assessed by the least significant difference method ( P < 0.05). We present experimental and theoretical studies on the antioxidant potential of the set of 22 phenolic acids with different models of (PDF) Antioxidant Activity of Selected Phenolic AcidsFerric Reducing Antioxidant Power Assay and QSAR Analysis of the Structural Features | Beata bikowska - Academia.edu A locked padlock) or https:// means youve safely connected to the .gov website. The best synergistic effect between caffeic acid and SAC based on DPPH, ABTS, and reducing power assays were observed at the ratio of 1:20, 1:35, and 1:70, respectively. Due to the high antioxidant activity of the tested fractions, their cytotoxic activity was evaluated via using preliminary brine shrimp lethality test and toward liver cancer cell line; HepG2 using Sulphorhodamine-B assay. Chemicals Antioxidant activity of BHT was measured by DPPH radical scavenging, iron reducing power and ABTS assay was shown in Table 2. The reducing power of various essential oils was conducted according to Oyaizu and Ferreira et al. FRAP assay by coulometric titration has been attractive as a highly sensitive, reliable, and simple test for reducing power evaluation. Another electrochemical version of the FRAP assay is the chronoamperometric measurement of antioxidant reducing power. Subsequently, the antioxidant activity was demonstrated by 2, 2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging assay, reducing power Thus far, various chemical tests coupled with highly sensitive and automated detection technologies have been used to evaluate antioxidant activity by special methods, like for instance scavenging activity against different types of free radicals or ROS, reducing power and metal chelation, among others. In this technique, ferric iron complexed to 2,4,6-tri(2-pyridyl)-1,3,5-triazine (TPTZ, Scheme 1 ) acts both as the oxidant and chromophore 2 [ 13 ]. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Share sensitive information only on official, secure websites. Electrochemical assay has been employed recently to study the activity of antioxidants; however, there is controversy as to whether reducing power fully characterizes the antioxidant activity. Antioxidant activity (DPPH radical activity, reducing power, SOA activity, total phenolic content and total flavonoid content) were evaluated in Indian variety of acerola and its squash. Reducing power assay The antioxidant activity of altered crude extracts was evaluated using the reducing power assay. However, in DPPH and ABTS assays, factors such as light, oxygen, and pH easily influence the color of the reactants mixture.